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dl10000 dna markers  (TaKaRa)


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    Structured Review

    TaKaRa dl10000 dna markers
    Double digestion of the tandem protein rND-32a. M: <t>DL10000</t> DNA Marker; ND-32a: recombinant plasmid pET-32a-ND; N: Negative control
    Dl10000 Dna Markers, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 7328 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dl10000+marker/T4+DNA+Ligase/pmc12870107-34-36-51
    Average 97 stars, based on 7328 article reviews
    dl10000 dna markers - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "Antigenicity analysis of the recombinant fusion proteins NlpI of Mannheimia haemolytica and DsbA of Pasteurella multocida"

    Article Title: Antigenicity analysis of the recombinant fusion proteins NlpI of Mannheimia haemolytica and DsbA of Pasteurella multocida

    Journal: BMC Veterinary Research

    doi: 10.1186/s12917-025-05233-6

    Double digestion of the tandem protein rND-32a. M: DL10000 DNA Marker; ND-32a: recombinant plasmid pET-32a-ND; N: Negative control
    Figure Legend Snippet: Double digestion of the tandem protein rND-32a. M: DL10000 DNA Marker; ND-32a: recombinant plasmid pET-32a-ND; N: Negative control

    Techniques Used: Marker, Recombinant, Plasmid Preparation, Negative Control

    Related Articles

    Transfection:

    Article Title: Construction of myostatin gene knockout C2C12 cell line and expression of related microRNA
    Article Snippet: The pX601 and pKO.1 plasmid carriers were purchased from Chengdu Chuanshikewei Biotechnology Co. Ltd. TRIzol reagent was purchased from Invitrogen; DNA glue recovery kit and cell genome DNA extraction kit were purchased from Shanghai Shenggong Biotechnology Co. Ltd.; HiScript II 1st Strand cDNA Synthesis Kit was purchased from Nanjing Nuoweizan Biotechnology Co. Ltd. .. The TurboFect transfection reagent was purchased from Promega; DH5α competent cells were purchased from Takara Bio Co. Ltd. High glucose DMEM medium, FBS, PBS and trypsin were purchased from Gibco Co. Ltd; Intermediate plasmid extraction kit was purchased from QIAGEN Corporation; Q5 DNA polymerase, Bsa endonuclease, BamH endonuclease, EcoR endonuclease, 10×NEBuffer 2, T4 DNA ligase and T7 endonuclease were purchased from NEB Co. Ltd. DL2000 Marker, DL10000 Marker, 50 bp DNA Ladder, SYBR Green kit purchased from Takara Co. Ltd; Murine Anti-MSTN antibody, murine anti-GAPDH antibody and horseradish peroxidase (HRP) -coupled Goat anti-mouse IgG were purchased from ABbot Anti-Trading Co. Ltd. Protease inhibitor, strong RIPA lysate and BCA protein quantitative detection kit were purchased from Biyuntian Biotechnology Co. Ltd.; CCK-8 kit was purchased from Dongren Chemical Technology (Shanghai) Co. Ltd.; Fluorescent dye PI was purchased from Beijing Zhuangmeng International Biological Gene Technology Co. Ltd. Design of sgRNA targeting sites in Mstn gene The MSTN encoding gene in mice (NM_010834. .. 3) was downloaded from NCBI (https://www.ncbi.nlm.nih.gov/) database, upload the Mstn exons sequence into sgRNA Design software (http://www.broadinstitute Page 4/17 .org/rnai/public/analysis-tools/sgrna-design).According to the score and exon 5 'region, two pairs of suitable sgRNAs were selected and named as sgRNA1-MSTN and sgRNA2-MSTN.

    Plasmid Preparation:

    Article Title: Construction of myostatin gene knockout C2C12 cell line and expression of related microRNA
    Article Snippet: The pX601 and pKO.1 plasmid carriers were purchased from Chengdu Chuanshikewei Biotechnology Co. Ltd. TRIzol reagent was purchased from Invitrogen; DNA glue recovery kit and cell genome DNA extraction kit were purchased from Shanghai Shenggong Biotechnology Co. Ltd.; HiScript II 1st Strand cDNA Synthesis Kit was purchased from Nanjing Nuoweizan Biotechnology Co. Ltd. .. The TurboFect transfection reagent was purchased from Promega; DH5α competent cells were purchased from Takara Bio Co. Ltd. High glucose DMEM medium, FBS, PBS and trypsin were purchased from Gibco Co. Ltd; Intermediate plasmid extraction kit was purchased from QIAGEN Corporation; Q5 DNA polymerase, Bsa endonuclease, BamH endonuclease, EcoR endonuclease, 10×NEBuffer 2, T4 DNA ligase and T7 endonuclease were purchased from NEB Co. Ltd. DL2000 Marker, DL10000 Marker, 50 bp DNA Ladder, SYBR Green kit purchased from Takara Co. Ltd; Murine Anti-MSTN antibody, murine anti-GAPDH antibody and horseradish peroxidase (HRP) -coupled Goat anti-mouse IgG were purchased from ABbot Anti-Trading Co. Ltd. Protease inhibitor, strong RIPA lysate and BCA protein quantitative detection kit were purchased from Biyuntian Biotechnology Co. Ltd.; CCK-8 kit was purchased from Dongren Chemical Technology (Shanghai) Co. Ltd.; Fluorescent dye PI was purchased from Beijing Zhuangmeng International Biological Gene Technology Co. Ltd. Design of sgRNA targeting sites in Mstn gene The MSTN encoding gene in mice (NM_010834. .. 3) was downloaded from NCBI (https://www.ncbi.nlm.nih.gov/) database, upload the Mstn exons sequence into sgRNA Design software (http://www.broadinstitute Page 4/17 .org/rnai/public/analysis-tools/sgrna-design).According to the score and exon 5 'region, two pairs of suitable sgRNAs were selected and named as sgRNA1-MSTN and sgRNA2-MSTN.

    Extraction:

    Article Title: Construction of myostatin gene knockout C2C12 cell line and expression of related microRNA
    Article Snippet: The pX601 and pKO.1 plasmid carriers were purchased from Chengdu Chuanshikewei Biotechnology Co. Ltd. TRIzol reagent was purchased from Invitrogen; DNA glue recovery kit and cell genome DNA extraction kit were purchased from Shanghai Shenggong Biotechnology Co. Ltd.; HiScript II 1st Strand cDNA Synthesis Kit was purchased from Nanjing Nuoweizan Biotechnology Co. Ltd. .. The TurboFect transfection reagent was purchased from Promega; DH5α competent cells were purchased from Takara Bio Co. Ltd. High glucose DMEM medium, FBS, PBS and trypsin were purchased from Gibco Co. Ltd; Intermediate plasmid extraction kit was purchased from QIAGEN Corporation; Q5 DNA polymerase, Bsa endonuclease, BamH endonuclease, EcoR endonuclease, 10×NEBuffer 2, T4 DNA ligase and T7 endonuclease were purchased from NEB Co. Ltd. DL2000 Marker, DL10000 Marker, 50 bp DNA Ladder, SYBR Green kit purchased from Takara Co. Ltd; Murine Anti-MSTN antibody, murine anti-GAPDH antibody and horseradish peroxidase (HRP) -coupled Goat anti-mouse IgG were purchased from ABbot Anti-Trading Co. Ltd. Protease inhibitor, strong RIPA lysate and BCA protein quantitative detection kit were purchased from Biyuntian Biotechnology Co. Ltd.; CCK-8 kit was purchased from Dongren Chemical Technology (Shanghai) Co. Ltd.; Fluorescent dye PI was purchased from Beijing Zhuangmeng International Biological Gene Technology Co. Ltd. Design of sgRNA targeting sites in Mstn gene The MSTN encoding gene in mice (NM_010834. .. 3) was downloaded from NCBI (https://www.ncbi.nlm.nih.gov/) database, upload the Mstn exons sequence into sgRNA Design software (http://www.broadinstitute Page 4/17 .org/rnai/public/analysis-tools/sgrna-design).According to the score and exon 5 'region, two pairs of suitable sgRNAs were selected and named as sgRNA1-MSTN and sgRNA2-MSTN.

    Marker:

    Article Title: Construction of myostatin gene knockout C2C12 cell line and expression of related microRNA
    Article Snippet: The pX601 and pKO.1 plasmid carriers were purchased from Chengdu Chuanshikewei Biotechnology Co. Ltd. TRIzol reagent was purchased from Invitrogen; DNA glue recovery kit and cell genome DNA extraction kit were purchased from Shanghai Shenggong Biotechnology Co. Ltd.; HiScript II 1st Strand cDNA Synthesis Kit was purchased from Nanjing Nuoweizan Biotechnology Co. Ltd. .. The TurboFect transfection reagent was purchased from Promega; DH5α competent cells were purchased from Takara Bio Co. Ltd. High glucose DMEM medium, FBS, PBS and trypsin were purchased from Gibco Co. Ltd; Intermediate plasmid extraction kit was purchased from QIAGEN Corporation; Q5 DNA polymerase, Bsa endonuclease, BamH endonuclease, EcoR endonuclease, 10×NEBuffer 2, T4 DNA ligase and T7 endonuclease were purchased from NEB Co. Ltd. DL2000 Marker, DL10000 Marker, 50 bp DNA Ladder, SYBR Green kit purchased from Takara Co. Ltd; Murine Anti-MSTN antibody, murine anti-GAPDH antibody and horseradish peroxidase (HRP) -coupled Goat anti-mouse IgG were purchased from ABbot Anti-Trading Co. Ltd. Protease inhibitor, strong RIPA lysate and BCA protein quantitative detection kit were purchased from Biyuntian Biotechnology Co. Ltd.; CCK-8 kit was purchased from Dongren Chemical Technology (Shanghai) Co. Ltd.; Fluorescent dye PI was purchased from Beijing Zhuangmeng International Biological Gene Technology Co. Ltd. Design of sgRNA targeting sites in Mstn gene The MSTN encoding gene in mice (NM_010834. .. 3) was downloaded from NCBI (https://www.ncbi.nlm.nih.gov/) database, upload the Mstn exons sequence into sgRNA Design software (http://www.broadinstitute Page 4/17 .org/rnai/public/analysis-tools/sgrna-design).According to the score and exon 5 'region, two pairs of suitable sgRNAs were selected and named as sgRNA1-MSTN and sgRNA2-MSTN.

    SYBR Green Assay:

    Article Title: Construction of myostatin gene knockout C2C12 cell line and expression of related microRNA
    Article Snippet: The pX601 and pKO.1 plasmid carriers were purchased from Chengdu Chuanshikewei Biotechnology Co. Ltd. TRIzol reagent was purchased from Invitrogen; DNA glue recovery kit and cell genome DNA extraction kit were purchased from Shanghai Shenggong Biotechnology Co. Ltd.; HiScript II 1st Strand cDNA Synthesis Kit was purchased from Nanjing Nuoweizan Biotechnology Co. Ltd. .. The TurboFect transfection reagent was purchased from Promega; DH5α competent cells were purchased from Takara Bio Co. Ltd. High glucose DMEM medium, FBS, PBS and trypsin were purchased from Gibco Co. Ltd; Intermediate plasmid extraction kit was purchased from QIAGEN Corporation; Q5 DNA polymerase, Bsa endonuclease, BamH endonuclease, EcoR endonuclease, 10×NEBuffer 2, T4 DNA ligase and T7 endonuclease were purchased from NEB Co. Ltd. DL2000 Marker, DL10000 Marker, 50 bp DNA Ladder, SYBR Green kit purchased from Takara Co. Ltd; Murine Anti-MSTN antibody, murine anti-GAPDH antibody and horseradish peroxidase (HRP) -coupled Goat anti-mouse IgG were purchased from ABbot Anti-Trading Co. Ltd. Protease inhibitor, strong RIPA lysate and BCA protein quantitative detection kit were purchased from Biyuntian Biotechnology Co. Ltd.; CCK-8 kit was purchased from Dongren Chemical Technology (Shanghai) Co. Ltd.; Fluorescent dye PI was purchased from Beijing Zhuangmeng International Biological Gene Technology Co. Ltd. Design of sgRNA targeting sites in Mstn gene The MSTN encoding gene in mice (NM_010834. .. 3) was downloaded from NCBI (https://www.ncbi.nlm.nih.gov/) database, upload the Mstn exons sequence into sgRNA Design software (http://www.broadinstitute Page 4/17 .org/rnai/public/analysis-tools/sgrna-design).According to the score and exon 5 'region, two pairs of suitable sgRNAs were selected and named as sgRNA1-MSTN and sgRNA2-MSTN.

    Protease Inhibitor:

    Article Title: Construction of myostatin gene knockout C2C12 cell line and expression of related microRNA
    Article Snippet: The pX601 and pKO.1 plasmid carriers were purchased from Chengdu Chuanshikewei Biotechnology Co. Ltd. TRIzol reagent was purchased from Invitrogen; DNA glue recovery kit and cell genome DNA extraction kit were purchased from Shanghai Shenggong Biotechnology Co. Ltd.; HiScript II 1st Strand cDNA Synthesis Kit was purchased from Nanjing Nuoweizan Biotechnology Co. Ltd. .. The TurboFect transfection reagent was purchased from Promega; DH5α competent cells were purchased from Takara Bio Co. Ltd. High glucose DMEM medium, FBS, PBS and trypsin were purchased from Gibco Co. Ltd; Intermediate plasmid extraction kit was purchased from QIAGEN Corporation; Q5 DNA polymerase, Bsa endonuclease, BamH endonuclease, EcoR endonuclease, 10×NEBuffer 2, T4 DNA ligase and T7 endonuclease were purchased from NEB Co. Ltd. DL2000 Marker, DL10000 Marker, 50 bp DNA Ladder, SYBR Green kit purchased from Takara Co. Ltd; Murine Anti-MSTN antibody, murine anti-GAPDH antibody and horseradish peroxidase (HRP) -coupled Goat anti-mouse IgG were purchased from ABbot Anti-Trading Co. Ltd. Protease inhibitor, strong RIPA lysate and BCA protein quantitative detection kit were purchased from Biyuntian Biotechnology Co. Ltd.; CCK-8 kit was purchased from Dongren Chemical Technology (Shanghai) Co. Ltd.; Fluorescent dye PI was purchased from Beijing Zhuangmeng International Biological Gene Technology Co. Ltd. Design of sgRNA targeting sites in Mstn gene The MSTN encoding gene in mice (NM_010834. .. 3) was downloaded from NCBI (https://www.ncbi.nlm.nih.gov/) database, upload the Mstn exons sequence into sgRNA Design software (http://www.broadinstitute Page 4/17 .org/rnai/public/analysis-tools/sgrna-design).According to the score and exon 5 'region, two pairs of suitable sgRNAs were selected and named as sgRNA1-MSTN and sgRNA2-MSTN.

    CCK-8 Assay:

    Article Title: Construction of myostatin gene knockout C2C12 cell line and expression of related microRNA
    Article Snippet: The pX601 and pKO.1 plasmid carriers were purchased from Chengdu Chuanshikewei Biotechnology Co. Ltd. TRIzol reagent was purchased from Invitrogen; DNA glue recovery kit and cell genome DNA extraction kit were purchased from Shanghai Shenggong Biotechnology Co. Ltd.; HiScript II 1st Strand cDNA Synthesis Kit was purchased from Nanjing Nuoweizan Biotechnology Co. Ltd. .. The TurboFect transfection reagent was purchased from Promega; DH5α competent cells were purchased from Takara Bio Co. Ltd. High glucose DMEM medium, FBS, PBS and trypsin were purchased from Gibco Co. Ltd; Intermediate plasmid extraction kit was purchased from QIAGEN Corporation; Q5 DNA polymerase, Bsa endonuclease, BamH endonuclease, EcoR endonuclease, 10×NEBuffer 2, T4 DNA ligase and T7 endonuclease were purchased from NEB Co. Ltd. DL2000 Marker, DL10000 Marker, 50 bp DNA Ladder, SYBR Green kit purchased from Takara Co. Ltd; Murine Anti-MSTN antibody, murine anti-GAPDH antibody and horseradish peroxidase (HRP) -coupled Goat anti-mouse IgG were purchased from ABbot Anti-Trading Co. Ltd. Protease inhibitor, strong RIPA lysate and BCA protein quantitative detection kit were purchased from Biyuntian Biotechnology Co. Ltd.; CCK-8 kit was purchased from Dongren Chemical Technology (Shanghai) Co. Ltd.; Fluorescent dye PI was purchased from Beijing Zhuangmeng International Biological Gene Technology Co. Ltd. Design of sgRNA targeting sites in Mstn gene The MSTN encoding gene in mice (NM_010834. .. 3) was downloaded from NCBI (https://www.ncbi.nlm.nih.gov/) database, upload the Mstn exons sequence into sgRNA Design software (http://www.broadinstitute Page 4/17 .org/rnai/public/analysis-tools/sgrna-design).According to the score and exon 5 'region, two pairs of suitable sgRNAs were selected and named as sgRNA1-MSTN and sgRNA2-MSTN.



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    Image Search Results


    Double digestion of the tandem protein rND-32a. M: DL10000 DNA Marker; ND-32a: recombinant plasmid pET-32a-ND; N: Negative control

    Journal: BMC Veterinary Research

    Article Title: Antigenicity analysis of the recombinant fusion proteins NlpI of Mannheimia haemolytica and DsbA of Pasteurella multocida

    doi: 10.1186/s12917-025-05233-6

    Figure Lengend Snippet: Double digestion of the tandem protein rND-32a. M: DL10000 DNA Marker; ND-32a: recombinant plasmid pET-32a-ND; N: Negative control

    Article Snippet: The bacterial genomic DNA extraction kit, product purification kit, plasmid extraction kit, ClonExpress Ultra One Step Cloning Kit V2, and 2× Taq Plus Master Mix II were purchased from Nanjing Novizan Biotechnology Co., Ltd.; DL2000 and DL10000 DNA Markers, T4 DNA ligase, and restriction endonucleases BamHI and XhoI were purchased from Takara Bio.

    Techniques: Marker, Recombinant, Plasmid Preparation, Negative Control